OSR-M405-TGuide FFPE DNA One-Step Kit

TGuide FFPE DNA One-Step Kit

TGuide FFPE DNA One-Step Kit
Cat. no. OSR-M405

Storage Conditions:
This kit can be stored at room temperature (15-30°C) under dry condition
for 12 months.

Product Description:
The TGuide FFPE DNA One-Step Kit is specifically designed to extract
genomic DNA from formalin-fixed, paraffin-embedded tissue samples in
conjunction with TGuide M16 Automated Nucleic Acid Extractor. The kit
adopts a one-step heating method to simultaneously perform paraffin
dissolution and tissue lysis, and does not contain harmful reagents
such as xylene. There are two extraction options for this kit: For small
amount samples, the lysis time is 2 hours; for large amount samples, it is
recommended to lyse the samples overnight for 16 hours. This kit utilizes
cellulose-embedded magnetic beads technology to efficiently extract
genomic DNA.
The genomic DNA extracted by this kit can be directly used for various
routine operations without further purification, including enzyme
digestion, PCR, library construction and Southern blot, etc.

Important Notes:

1. 1-5 rolls of the FFPE samples with the roll thickness less than 5 µm can
   be extracted by this kit. For the samples with the surface area larger
   than 300 mm
   2
   , please equally cut the sample into 4 pieces.
2. For the first time applied samples, it is recommended to use no more
   than 1 roll of the sample amount, and cut the sample before extracting.
3. Sula Oil is a deparaffinization buffer. 20 mg paraffin can be dissolved in
   500 µl Sula Oil.

Features:

1. Deparaffinization in the machine:The deparaffinization and extraction
   can be automatically completed by putting the untreated FFPE samples
   in to the reagent cartridge and add in Sula Oil and proteinase K, without
   the need for manually deparaffinizing.
2. Reliable results:The extracted genomic DNA is free from the
   contamination of RNA or proteins, and can be directly used for PCR or
   qPCR applications.
3. Safe and non-toxic: No organic solvents such as phenol and chloroform
   which are harmful to the human body are needed for the operation.

Protocol:

1. Prepare the FFPE samples as recommended in the Important Notes.
2. Add 500 μl Sula Oil, 20 μl proteinase K and the prepared FFPE samples
   to the bottom of heating well 1, and tighten the ThermoStable Cap.
   Note: If the FFPE sample size is too large (>300 mm
   2
   ), cut the sample
   evenly into 4 pieces (see Important Notes). Please make sure to add
   the sample to the bottom of the heating well 1 to prevent the sample
   from getting stuck on the ThermoStable Cap.
3. Place the collection tube in the well labeled “1” of the T-rack, and
   place the pipette tips and the tip cap in the wells labeled “2” and “3”.
   Run the cartridge code 405 program (FFPE DNA extraction), set the
   deparaffinization time (2 h or 16 h), and select the final elution volume.
   Note: If higher yield is required, it is recommended to use the 16 h
   deparaffinization time. However, the long-time treatment might cause
   DNA degradation.

OSR-M405-TGuide FFPE DNA One-Step Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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