RNAprep Pure Cell/Bacteria Kit – ( Tiangen Gdp430 )( Go2biotech.com – Distribution for Reagents and instruments )

For purification of high-quality total RNA from cells and bacteria

RNAprep Pure Cell/Bacteria Kit – ( Tiangen Gdp430 ) Catalog number / packaging

Description
The RNAprep Pure Cell/Bacteria Kit provides a fast, simple and cost-effective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. The kit includes RNase-Free Spin Column CR3 for purifying high quality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes with high-purity and is free of protein and genomic DNA contamination.

Required Reagents
β-mercaptoethanol, ethanol, lysozyme (optional for bacterial RNA extraction)

Features
■ Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.

■ Unique DNase I minimizes genomic DNA contamination.

■ Unique RNase-Free Filtration Columns CS eliminates other contaminations.

■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.

■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

Applications
■ RT-PCR.

■ Northern Blot, Dot Blot.

■ Real-Time PCR.

■ Chip analysis.

■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

Storage
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

Other reagents could be stored at room temperature (15-25℃)

RNAprep Pure Cell/Bacteria Kit – ( Tiangen Gdp430 ) Publication

• Acetylation of xenogeneic silencer H-NS regulates biofilm development through the nitrogen homeostasis regulator in ShewanellaNucleic Acids Research2024-04-12 Read ArticleTotal RNA was extracted; DNase-treated; and purified with an RNAprep Pure Cell/Bacteria Kit (Tiangen; Beijing; China).

• Regulation of quorum sensing activities by the stringent response gene rsh in sphingomonads is species-specific and culture condition dependentFrontiers in Microbiology2024-04-04 Read Article.. their total RNA was extracted with RNAprep pure Cell/Bacteria Kit (Tiangen Biotech; Beijing; China) according to the manufacturer??s

• ApoE Mimetic Peptide COG1410 Kills Mycobacterium smegmatis via Directly Interfering ClpC??s ATPase ActivityAntibiotics2024-03-19 Read Article.. was extracted using RNAprep Pure Cell/Bacteria Kit (TIANGEN (Beijing; China); Cat. No. DP430); which was converted to cDNA by reverse

• Matrine reverses the resistance of Haemophilus parasuis to cefaclor by inhibiting the mutations in penicillin-binding protein genes ( ftsI and mrcA )Frontiers in Microbiology2024-03-15 Read Article.. RNA was extracted using a RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech Co. Ltd.; Shanghai; China); according to

• Programmable marine bacteria catalyze the valorization of lignin monomersbioRxiv2024-03-14 Read ArticlePrePrint: Total RNA was extracted using RNAprep Pure Cell/Bacteria Kit (TIANGEN) and reverse-transcribed into cDNA using PrimeScript???

RNAprep Pure Cell/Bacteria Kit – ( Tiangen Gdp430 ) Manual

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

RNAprep Pure Cell/Bacteria Kit – ( Tiangen Gdp430 )

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