RNAprep Pure Hi-Blood Kit

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For purification of high-quality and stable total RNA from blood

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• StorageBuffer RLH with β-mercaptoethanol can be stored at 4℃ for one month. DNase I, Buffer RDD, should be stored at 2-8℃.Other reagents could be store at room temperature (15-25℃)
• DescriptionThe RNAprep Pure Hi-Blood Kit efficiently extracts total RNA from fresh whole blood and blood with multiple anticoagulants from different species. The silicon matrix material used in the adsorption column is a unique new material developed by TIANGEN, which efficiently and specifically adsorbs RNA, and removes impurity proteins to the utmost extent. The extracted RNA can be used in various downstream experiments such as RT-PCR, RT-qPCR, chip analysis, high-throughput sequencing, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.
• Required Reagentsβ-mercaptoethanol, ethanol
• Features■ Suitable for fresh whole blood of different species, easy to operate.■ Equipped with RNase-Free Filtration Column CS to effectively remove impurities.■ The specifically formulated buffer can ensure efficient and stable RNA extraction for a variety of downstream experiments.■ Safe and reliable operation, no phenol/chloroform extraction required.
• ApplicationRT-PCR, Northern Blot, RT-qPCR, chip analysis, high-throughput sequencing, PolyA screening, RNase protection analysis, in vitro translation, etc.

Publication

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• A novel homozygous splice-site mutation of JK gene leads to Jk(a-b-) phenotype.Transfusion medicine (Oxford, England)2024-03-19 Read ArticleAbstract: Funding information National Natural Science Foundation of China; Grant/Award Number: 81970168 Abstract Objectives: This study aimed to investigate the molecular mechanism of the Jk(a-b-) phenotype in a Chinese transfusion patient.. Background: Many different mutation types relating to Jk(a-b-) phenotype have been reported.. However; the splice-site mutation is relatively rare and the related functional verification is lacking.
• Identification of key genes increasing susceptibility to atrial fibrillation in nonalcoholic fatty liver disease and the potential mechanisms: mitochondrial dysfunction and systemic inflammationFrontiers in Pharmacology2024-03-14 Read ArticleTotal RNA from blood was extracted using a high-performance blood total RNA extraction kit (DP443; TIANGEN) following the standard protocol.
• PLCG2 and IFNAR1: The Potential Biomarkers Mediated by Immune Infiltration and Osteoclast Differentiation of Ankylosing Spondylitis in the Peripheral BloodMediators of Inflammation2024-01-01 Read ArticleThe RNAprep Pure Hi-Blood Kit (Tiangen Biotech; China) was used to perform the reverse transcription of the extracted RNA.
• Absence of toll-like receptor 7 ameliorates survival and reduces intestinal injury in mice after Clostridium difficile infection.Microbes and infection2023-12-20 Read ArticleAbstract: Clostridioides difficile (CD) is a major cause of antibiotic-associated diarrhea and pseudomembranous enteritis.. C. difficile infection (CDI) is increasingly present in the community and represents a significant burden on the healthcare system.. Identification of novel immune-based therapeutic targets from a better understanding of their molecular pathogenesis is urgently required.
• The Potential Relevance of PnDREBs to Panax notoginseng Nitrogen Sensitiveness.Biochemical genetics2023-11-24 Read ArticleAbstract: The dehydration response element-binding (DREB) transcription factor is a subfamily of AP2/ERF.. It actively responds to various abiotic stresses in plants.. As one of the representative plants; Panax notoginseng is sensitive to Nitrogen (N).

RNAprep Pure Hi-Blood Kit

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