RNAprep Pure Micro Kit
.. 248 / Publication
For purification of high-quality total RNA from micro amount of tissues or cells
Catalog number / packaging
Mat. No | Ref. No | No. of preps |
4992859 | Gdp420 | 50 |
- StorageDNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃Other reagents could be store at room temperature (15-25℃)The function of carrier RNA: A special carrier RNA is provided in the kit. When RNA is extracted from a very small amount of sample, it is recommended to add Carrier RNA in the system, which helps to improve the binding of RNA to adsorption column.
- DescriptionThe RNAprep Pure Micro Kit uses a highly efficient, nucleic acid-specific centrifugal adsorption column and a unique buffer system to rapidly extract total RNA from a wide range of different types of microsamples. This kit includes Carrier RNA, which can easily capture trace nucleic acids from the system. The extraction of RNA by this kit is convenient, rapid and reproducible with high yield. The reaction can be completed within 30-40 minutes. The kit can selectively remove all RNA that is 200 nt. The extracted total RNA is extremely pure and free of DNA and protein contamination.The RNAprep Pure Micro Kit uses a highly efficient, nucleic acid-specific centrifugal adsorption column and a unique buffer system to rapidly extract total RNA from a wide range of different types of microsamples. This kit includes Carrier RNA, which can easily capture trace nucleic acids from the system. The extraction of RNA by this kit is convenient, rapid and reproducible with high yield. The reaction can be completed within 30-40 minutes. The kit can selectively remove all RNA that is 200 nt. The extracted total RNA is extremely pure and free of DNA and protein contamination.
- Required Reagentsβ-mercaptoethanol, ethanol
- Features■ Capable of purifying high-quality RNA from trace amount samples, such as micro-dissected tissue, fibrous tissue and cells.■ Unique DNase I minimizes genomic DNA contamination.■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.
- Applications■ RT-PCR.■ Northern Blot, Dot Blot.■ Real-Time PCR.■ Chip analysis.■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.
Publication
.. 248 / Publication
Sort by
Impact Factor
Journals
–
- A Bursaphelenchus xylophilus effector BxICD1 inducing plant cell death, concurrently contributes to nematode virulence and migrationFrontiers in Plant Science2024-02-28 Read Article.. Total RNA was extracted using the RNAprep Pure Micro Kit (DP420; TianGen Biotech); and first-strand cDNA was synthesized from
- Glucocorticoids increase adiposity by stimulating Kr??ppel-like factor 9 expression in macrophagesNature Communications2024-02-08 Read ArticleATMs RNA was collected from the sorted cells via RNAprep pure Micro Kit (TIANGEN).
- Human Fallopian Tube-Derived Organoids with TP53 and RAD51D Mutations Recapitulate an Early Stage High-Grade Serous Ovarian Cancer Phenotype In Vitro.International journal of molecular sciences2024-01-24 Read ArticleRNA extraction was performed by using an RNAprep Pure Micro Kit (DP420; Tiangen; Beijing; China).
- KAS-seq profiling captures transcription dynamics during oocyte maturationJournal of Ovarian Research2024-01-24 Read Article.. of 50 oocytes from the specified experimental group; employing the RNAprep Pure Micro Kit (TIANGEN DP420).
- The Effects of Nine Compounds on Aldehyde-Oxidase-Related Genes in Bactrocera dorsalis (Hendel).Genes2024-01-24 Read Article.. trace sample total RNA extraction kit was used (DP420; TIANGEN BIOTECH Co.; Ltd.; Beijing; China); as well as a Quant
RNAprep Pure Micro Kit
Need more info ? Contact us anytime. We’re here:
Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
Address: Rm 4L, Golden Century Building, Shennan Av. 6033#, Shenzhen, China