RNAsimple Total RNA Kit – ( Tiangen GDP419 )( Go2biotech.com – Distribution for Reagents and instruments )

For the high-efficient total RNA extraction using the widely used centrifugal column

RNAsimple Total RNA Kit – ( Tiangen GDP419 ) Catalog number / packaging

Mat. NoRef. NoNo. of preps
4992858GDP41950


Description
The RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate/phenol method. The Buffer RZ specifically designed by TIANGEN can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.
This kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5×106 cells at a time and can process a large number of different samples simultaneously. The reaction can be completed in less than one hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.

Required Reagents
Ethanol, chloroform

Features
■ The high-purity ready-to-use RNA is suitable for sensitive downstream applications.

■ Wide applications: The purified RNA can be applied to various experimental samples.

■ The experiment can be completed in 1 hour with simple operations.

Applications
■ RT-PCR

■ Northern Blot, Dot Blot.

■ Real-Time PCR

■ PolyA Screening, in vitro translation, RNase protection analysis, molecular cloning.

Storage
Store at room temperature (15-25℃)

Buffer RZ should be stored at 2-8℃ away from light

RNAsimple Total RNA Kit – ( Tiangen GDP419 ) Publication

  • Ameliorative Potential of (-) Pseudosemiglabrin in Mice with Pilocarpine-Induced Epilepsy: Antioxidant, Anti-Inflammatory, Anti-Apoptotic, and Neurotransmission ModulationInternational Journal of Molecular Sciences2023-06-28 Read Article.. brain tissues utilizing a total RNA extraction kit (RNAsimple Total RNA Kit; Tiangen Biotech; Beijing; China; Cat. #
  • IQCH regulates spermatogenesis by interacting with CaM to promote RNA-binding proteins?? expressionbioRxiv2023-06-26 Read ArticlePrePrint: .. (n=3) and the Iqch KO mice (n=3) using the RNAsimple
  • Shikonin Alleviates Gentamicin-Induced Renal Injury in Rats by Targeting Renal Endocytosis, SIRT1/Nrf2/HO-1, TLR-4/NF-??B/MAPK, and PI3K/Akt CascadesAntibiotics2023-04-28 Read ArticleBp65; p38 MAPK; p-AKT; and PI3K; quantitative real-time polymerase chain reaction was utilized. Total RNA was isolated from the renal tissues using a total RNA extraction kit (RNAsimple Total RNA Kit; Tiangen Biotech; Beijing; China; Cat. #. 4992858) according to the manufacturer??s instructions. Reverse transcription was performed using an iScript cDNA Synthesis Kit (Bio-Rad; Hercules; CA; USA; Cat
  • Shikonin Alleviates Gentamicin-Induced Renal Injury in Rats by Targeting Renal Endocytosis, SIRT1/Nrf2/HO-1, TLR-4/NF-??B/MAPK, and PI3K/Akt Cascades.Antibiotics (Basel, Switzerland)2023-04-28 Read Articleantitative real-time polymerase chain Antibiotics 2023; 12; 826 17 of 20 reaction was utilized. Total RNA was isolated from the renal tissues using a total RNA extraction kit (RNAsimple Total RNA Kit; Tiangen Biotech; Beijing; China; Cat. #. 4992858) according to the manufacturer??s instructions. Reverse transcription was performed using an iScript cDNA Synthesis Kit (Bio-Rad; Hercules; CA; USA; Cat

 RNAsimple Total RNA Kit – ( Tiangen GDP419 ) Manual

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

RNAsimple Total RNA Kit – ( Tiangen GDP419 )

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