RNAstore Reagent-Tiangen OSE- ( Go2biotech.com – Distribution for Reagents and instruments )

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Non-freezing reagent for protecting the integrity of sample RNA

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• StorageStore at room temperature (15-25℃ )
• DescriptionThe RNAstore Reagent is a liquid, non-toxic tissue preservation reagent. It rapidly penetrates into tissue cells and protects non-frozen cells from RNA in situ by efficiently inhibiting RNase activity, making it more suitable for analyzing tissue gene expression profiling.For tissue sample storage, the tissue can be quickly immersed in the RNAstore for storage to avoid the degradation of the RNA, so that the sample does not have to be processed immediately or frozen in liquid nitrogen.RNAstore Reagent can be widely used in a variety of vertebrate samples, including brain, heart, kidney, spleen, liver, lung and thymus.
• Features■ Storage conditions: This kit can be stored for 1 week at room temperature, 1 day at 37°C, and at least 1 month at 4°C. For tissue samples, immerse at 4°C overnight, and transfer to -20°C or -80°C for long-term storage.■ Repeated freezing and thawing: Tissue frozen at -20°C or -80°C can be freeze-thawed for 20 times without affecting the quality of RNA extraction.■Downstream applications: After removing the sample from the RNAstore Reagent, total RNA can be extracted by TIANGEN’s TRNzol, RNAprep Pure, RNAsimple reagents and kits.
• Important Notes■ RNAstore is only suitable for fresh tissue samples.■ RNAstore is not suitable for plant tissue samples.■ The amount ratio of the tissue samples and RNAstore Reagent should be at least 1:10 (eg for 100 mg tissue, at least 1 ml of RNAstore is required).■ The thickness of each side of the sample should not exceed 0.5 cm to ensure that the RNAstore can penetrate quickly into the tissue.

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• HIF-1-mediated regulation of LDH gene unravels key insights into MCDV-1 pathogenesis in mud crabs Scylla paramamosain.Developmental and comparative immunology2023-12-29 Read ArticleAbstract: 22 Hypoxia-inducible factors -1 (HIF-1) is a crucial transcription factor that 23 regulates the expression of glycolytic genes.. Our previous study proved 24 that the Mud crab dicistrovirus-1 (MCDV-1) can induce aerobic glycolysis 25 that favors viral replication in mud crab Scylla paramamosain.. However; 26 the role of HIF-1 on key glycolytic genes during the MCDV-1 infection 27 has not been examined.
• Poly(I:C) induces anti-inflammatory response against secondary LPS challenge in zebrafish larvae.Fish & shellfish immunology2023-12-12 Read ArticleAbstract: Poly(I:C) is known as an agonist of the TLR3 receptor which could prime inflammation and elicit the host immune response; which is widely applied as adjuvant or antivirus treatment.. However; the negative effects of poly (I:C) on regulating immune response to protect the host from inflammatory diseases remain largely unknown.. Here; we establish an in vivo model to pre-treat zebrafish larvae with poly(I:C) at 2 dpf; then challenge them with LPS at 6 dpf; and find that poly(I:C) training could significantly alleviate the LPS challenge-induced septic shock and inflammatory phenotypes.
• Combined effects of microplastics and antibiotic-resistant bacteria on Daphnia magna growth and expression of functional genes.The Science of the total environment2023-11-15 Read ArticleAbstract: ?? The colonization of antibiotic-resistant bacteria (ARB) on microplastics is. Science of the Total Environment 905 (2023) 166880 Available online 12 September 2023 0048-9697/?? 2023 Elsevier B.V. All rights reserved.. Combined effects of microplastics and antibiotic-resistant bacteria on Daphnia magna growth and expression of functional genes Xiaofeng Chen 1; Xiaocui Wang 1; Yuanyin Huang; Ziying Zhu; Tianmu Li; Zeming Cai; Minqian Li; Han Gong *; Muting Yan * College of Marine Sciences; South China Agricultural University; Guangzhou 510641; China H I G H L I G H T S G R A P H I C A L A B S T R A C T ?? The colonization of antibiotic-resistant bacteria (ARB) on microplastics is investigated.
• Transcriptome Analysis of the Spleen Provides Insight into the Immune Regulation of GCRV Resistance in Grass Carp (Ctenopharyngodon idella).Marine biotechnology (New York, N.Y.)2023-08-16 Read ArticleAbstract: Grass carp (Ctenopharyngodon idella) is one of the most economically important fish in China; and its production is commonly lost due to GCRV infection.. To understand the molecular mechanism of GCRV resistance in grass carp; we compared the spleen transcriptome of the GCRV-resistant and susceptible individuals under GCRV infection (Res-Sus) and the GCRV-resistant individuals under different conditions of injection with GCRV and PBS (Res-Ctl).. A total of 87.56 GB of clean data were obtained from 12 transcriptomic libraries of spleen tissues.
• NF-??B target TFAM to modulate mtDNA copies involved in lung injury of mice induced by radonEurope PMC2023-07-24 Read Article.. ..Beijing Chemical Reagent Company); RNAstore Sample Preservation Solution (China; Tiangen Biochemical Technology Co.; Ltd.);..

RNAstore Reagent

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