SuperReal PreMix Plus (SYBR Green）-GFP205

.. 2083 / Publication

Dual-enzyme family star products with outstanding stability and specificity

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• StorageStore at -20℃.
• DescriptionSuperReal PreMix Plus is a special reagent for Real-Time PCR developed on the basis of SuperReal PreMix. It is simple and convenient, with unique two-component HotStart DNA polymerase, optimized buffer system with original H-bond factor and ROX packaged separately. Due to the careful optimization of buffer, the generation of primer dimer and nonspecific amplification can be more effectively reduced, ensuring higher amplification specificity and wide template adaptability.
• Features■ Strong specificity: The buffer has a unique equilibrium system of K⁺ and NH⁴⁺ and an original H-Bond factor. The reaction system is upgraded and has higher amplification specificity.■ High sensitivity: Able to quantitatively detect the expression level of transcripts with low abundance.■ Good repeatability: The experimental results are stable and have good repeatability.■ Simple and fast: Only template, primer and ddH₂O need to be added in the premix to carry out real-time PCR reaction.■ ROX correction: ROX dyes packaged separately are more flexible to use and can ensure more accurate results.■ Widely applicable: It is widely applicable to experiments such as gene expression analysis and nucleic acid detection by SYBR Green method on various real-time PCR instruments such as ABI, Stratagene, Roche, Bio-Rad and Eppendorf.

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.. 2083 / Publication

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• UV-C radiation promoted flavonoid synthesis during early healing in potato tuber wounds with the possible involvement of ABA and related transcription factor regulationPostharvest Biology and Technology2024-03-01 Read ArticleAbstract: UV-C radiation has been widely proven to promote flavonoid synthesis in fruit and vegetables as an environmentally friendly treatment.. However; it is unknown whether UV-C promotes flavonoid synthesis in potato tuber wounds during early healing and its possible regulatory mechanism.. In this study; UV-C radiation increased the gene expression and activities of zeaxanthin epoxidase (ZEP) and 9-cis-epoxycarotenoid dioxygenase (NCED); and increased ABA levels.
• Transcriptional regulation of SARS-CoV-2 receptor ACE2 by SP1eLife2024-02-20 Read Article.. RT-qPCR analysis was performed using SuperReal PreMix Plus (SYBR Green) (FP205; TIANGEN) on a Roche LightCycler 480
• Improvement of ribonucleic acid production in Cyberlindnera jadinii and optimization of fermentation mediumAMB Express2024-02-15 Read Article.. reaction (qPCR) was conducted using SuperReal PreMix Plus (SYBR Green) (Tiangen Biotech; Beijing; China) and the CFX96
• DNA Methylome Regulates Virulence and Metabolism in Pseudomonas syringaebioRxiv2024-02-12 Read ArticlePrePrint: The assay was performed by SuperReal Premix Plus (SYBR Green) Kit (Tiangen Biotech) according to the manufacturer??s instruction.
• MUC1-EGFR crosstalk with IL-6 by activating NF-??B and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinomaAnnals of Medicine2024-02-07 Read ArticleThe SuperReal PreMix Plus (TIANGEN; Beijing; China) was used to amplify the target genes in the

SuperReal PreMix Plus (SYBR Green）