go2biotech – Reagents and instruments for immunology, cell biology and molecular biology.
Catalog number / packaging
Mat. No | Ref. No | No. of preps |
4992997 | DP810 | 24 preps |

- StorageThe kit can be stored for 12 months at room temperature (15-25°C). For long term storage, please store at 2-8°C. If precipitate has formed under the storage condition of 2-8°C, place the solution at room temperature for a period of time before use, or preheated in a 37°C water bath for 10 min if necessary to dissolve the precipitate.
- IntroductionThe kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality circulating nucleic acid from samples such as serum/plasma. The unique embedded magnetic beads have strong affinity for nucleic acid under certain conditions, and when the conditions change, the magnetic beads will release adsorbed nucleic acid, thus achieving the purpose of fast separation and purification of nucleic acid. The whole process is safe and convenient, with high yield of free nucleic acid extraction, high purity and stable and reliable quality, and is suitable for meet various downstream detection experiments and NGS analysis.
- Features1. The kit can be perfectly matched with the 24 flux module of TGuide S96 Automated Nucleic Acid Extractor to process the extraction of circulating nucleic acid from large-volume samples.2. The products obtained by the kit meet the requirements of downstream detection experiments and NGS analysis.
Publication
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- Application of mNGS in the study of pulmonary microbiome in pneumoconiosis complicated with pulmonary infection patients and exploration of potential biomarkersFrontiers in Cellular and Infection Microbiology2023-07-21 Read Articlehen leave at 4??C for 5??min to lower the reaction temperature. Transfer the sample to a sterile test tube and centrifuge briefly followed by DNA extraction using the TIANamp Magnetic DNA Kit (DP710-t2; Tiangen; China) according to the manufacturer??s protocol. Sputum was liquefied by 0.1% DTT (dithiothreitol) for 20??min at 56??C before extraction. The QIAamp Viral RNA Mini Kit (Qiagen) was used to ext
- Metagenomic surveillance and comparative genomic analysis of Chlamydia psittaci in patients with pneumoniaFrontiers in Microbiology2023-05-30 Read Articleadult community-acquired pneumonia ( Qu and Cao; 2016 ). Samples were processed through 10;000 g centrifugation for 5??min; and the pellets were collected for nucleic acid extraction using DP710-T2A (TIANGEN; China). Approximately 10??ng DNA was used for library construction with the Hieff NGS OnePot Pro DNA Library Prep Kit (Yeasen Biotech.; China). Using the mode of single-end 76-bp reads; the li
- Evaluation of the metagenomic next-generation sequencing performance in pathogenic detection in patients with spinal infectionFrontiers in Cellular and Infection Microbiology2022-10-27 Read Articleplementary Table??1 ) were applied to the clinical microbial culture in our hospital. In the mNGS analysis; DNA was extracted directly from tissue and (or) pus using the TIANamp Maxi DNA Kit (DP710; Tiangen Biotech; Beijing; China); following the manufacturer??s standard protocols. The extracted DNA was fragmented ultrasonically to yield 200-300bp fragments. DNA libraries were constructed through
- Characteristics of Lung Microbiota in Children’s Refractory Mycoplasma pneumoniae Pneumonia Coinfected with Human Adenovirus BThe Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien des Maladies Infectieuses et de la Microbiologie M??dicale2022-01-17 Read Article20??min and then placed at 4??C for 5??min. The sample was transferred into a sterile 5??ml tube; followed by brief centrifugation; and the DNA was extracted using the TIANamp Magnetic DNA kit (DP710-t2; Tiangen; China); according to the manufacturer’s protocol. A no-template control (NTC) was performed for PCR. The quantity was assessed using the Qubit 2.0 fluorometer (Thermo Fisher Scientific; USA);
- SEPT6 _ TRIM33 Gene Fusion and Mutated TP53 Pathway Associate With Unfavorable Prognosis in Patients With B-Cell LymphomasFrontiers in Oncology2021-12-01 Read Articlesity in accordance with the Declaration of Helsinki. All patients provided written informed consent. Total DNAs from blood samples were extracted using the Magnetic Serum/Plasma DNA Maxi Kit (DP710; TIANGEN Biotechnology; China); following the manufacturer??s instructions. The extracted cfDNA samples were quantified using Qubit dsDNA HS Assay Kit (Invitrogen) and sheared into 150 to 200 bp fragmen
TGuide S96 Circulating DNA Maxi Kit
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
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