TIANamp Bacteria DNA Kits-Tiangen-GDP302 ( Go2biotech.com – Distribution for Reagents and instruments )

Rapid extraction of high quality genomic DNA from various Gram-negative, Gram-positive bacteria.

Catalog number / packaging

TIANamp Bacteria DNA Kits-Tiangen-GDP302  Manual 

  • StorageRoom temperature (15-25℃)
  • DescriptionThe TIANamp Bacteria DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system for both genomic DNA extraction from Gram-negative and Gram-positive bacteria. The kit can also be used for the extraction of pathogenic bacteria (microorganisms) of food, such as Staphylococcus aureus, cholera sclerotia, hemorrhagic Escherichia coli O157:H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. 10-40 μl of pure genomic DNA can be rapidly extracted and purified from 1-5 ml of bacterial culture medium, and the obtained genomic DNA can be directly used in molecular biology experiments such as PCR template, restriction enzyme digestion and Southern blot.
  • Required ReagentsLysozyme (Gram-positive bacteria), ethanol, lysozyme buffer (20 mM Tris, pH8.0; 2 mM Na2-EDTA, 1.2% Triton-100)
  • Features■ Simple and fast: High purity genomic DNA of Gram-negative bacteria can be obtained within 1 hour■ Excellent quality: The purified DNA can be directly used in downstream molecular experiments such as PCR, restriction endonuclease digestion, Southern blotting, etc.
  • Applications■ Genome extraction of Gram-negative bacteria■ Genome extraction of Gram-positive bacteria■ Genome extraction of pathogenic bacteria in food
  • DNA Extraction Yield From Various Plant Tissues:
    Note: The DNA extraction amount may vary depending on the bacteria types and culture time, etc. Gram positive bacteria require special  treatments such as lysozyme for lysing, and the genomic DNA extraction can be performed according to the procedures of Gram-negative bacteria.

Publication

  • Mobile genetic elements facilitate the transmission of antibiotic resistance genes in multidrug-resistant Enterobacteriaceae from duck farmsFood Science and Human Wellness2024-03-01 Read ArticleBacterial DNA was extracted using Tianamp Bacteria DNA Kit (Tiangen biochemical technology (Beijing) Co.; Ltd.) and stored at -20 ??C.
  • Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrierJournal of Translational Medicine2024-02-17 Read Article.. from mouse fecal sample using the TIANamp Bacteria DNA Kit (Tiangen Biotech (Beijing) Co.; Ltd.; Beijing; China) in
  • Molecular characterization of extensively drug-resistant hypervirulent Pseudomonas aeruginosa isolates in ChinaAnnals of Clinical Microbiology and Antimicrobials2024-02-12 Read Article.. was extracted from XDR-hvPA strains using TIANamp Bacteria DNA Kit (Tiangen Biochemical Technology Co.; Ltd; Beijing) for
  • DNA Methylome Regulates Virulence and Metabolism in Pseudomonas syringaebioRxiv2024-02-12 Read ArticlePrePrint: The genomic DNA of the strains was extracted using a TIANamp Bacteria DNA kit (Tiangen Biotech); using the manufacturer??s standard protocols.
  • Taurine Regulates the Expression of Interleukin ???17/10 and Intestinal Flora and Protects the Liver and Intestinal Mucosa in a Nonalcoholic Fatty Liver Disease Rat ModelDiabetes, Metabolic Syndrome and Obesity2024-02-09 Read Article.. DNA was obtained using the TIANamp Bacteria DNA Kit (TIANGEN; Inc.) as the standard.

TIANamp Bacteria DNA Kits


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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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