TIANprep Rapid Mini Plasmid Kit

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For fast purification of plasmid DNA of molecular biology grade by alkaline lysis technique

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• StorageRoom temperature (15-25℃ )
• DescriptionThe TIANprep Rapid Mini Plasmid Kit is optimized from traditional alkaline lysis technology, by which high-quality plasmid DNA could be purified within 8 minutes. The new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt. The material which the silica membrane is made of is unique, highly efficient and highly specific. This kit is designed for purification of up to 24 μg plasmid DNA from 1-4 ml overnight cultures of E.coli. The plasmid DNA purified by this kit can be applied in various regular experiments, such as enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro transcription and the transfection of the common sub-culture cells, etc.
• Required ReagentsEthanol
• Features■ Fast: Simple procedure with minimal steps to purify high pure plasmid DNA within only 8 min.■ High efficiency: Could purify more than 85% plasmid DNA from E.coli.■ The color indicator TIANRed was supplied to control and remind every process during the experiment to ensure the high efficiency of plasmid purification.

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• Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae.Cell reports2024-04-01 Read Article.. K1622 Universal DNA Purification Kit TIANGEN Cat# DP214 TIANprep Rapid Mini Plasmid Kit TIANGEN Cat# DP105 SV Total
• Regulation of the pleiotropic transcriptional regulator CodY on the conversion of branched-chain amino acids into branched-chain aldehydes in Lactococcus lactisApplied and Environmental Microbiology2023-11-09 Read ArticlePlasmid DNAs of E. coli were extracted using the TIANprep Rapid Mini plasmid Kit (Tiangen) according to the manufacturer??s instructions.
• Supplementing maternal diet with milk oligosaccharides and probiotics helps develop the immune system and intestinal flora of offspringsFood Science & Nutrition2023-08-16 Read Article.. kit was purchased from Takara Co.; Ltd. TIANprep rapid mini plasmid kit and fecal DNA purification kit were purchased
• Bacteroides uniformis -induced perturbations in colonic microbiota and bile acid levels inhibit TH17 differentiation and ameliorate colitis developmentsNPJ Biofilms and Microbiomes2023-08-14 Read Articlet to AuGCT DNA-SYN Biotechnology Ltd. for sequencing. The results were compared using the NCBI tool BLAST ( www.ncbi.nih.gov/BLAST ). The standard plasmids were extracted using the Plasmid kit (DP105; Tiangen; China); and the standard curve was plotted at a 10-fold concentration gradient dilution. Bu-specific primers were used for qPCR amplification; and the bacterial copy number was calculated bas
• Bacteroides uniformis -induced perturbations in colonic microbiota and bile acid levels inhibit TH17 differentiation and ameliorate colitis developmentsNPJ Biofilms and Microbiomes2023-08-14 Read Articlet to AuGCT DNA-SYN Biotechnology Ltd. for sequencing. The results were compared using the NCBI tool BLAST ( www.ncbi.nih.gov/BLAST ). The standard plasmids were extracted using the Plasmid kit (DP105; Tiangen; China); and the standard curve was plotted at a 10-fold concentration gradient dilution. Bu-specific primers were used for qPCR amplification; and the bacterial copy number was calculated bas

TIANprep Rapid Mini Plasmid Kit

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