TIANScriptⅡ RT Kit-GKR107

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Suitable for templates with complex secondary structures and efficient synthesis of long-chain cDNA

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• StorageStore at -20°C.
• DescriptionThe kit provides all reagents for synthesizing the first strand of cDNA, wherein TIANScript II RTase is an improved Moloney murine leukemia virus reverse transcriptase (M-MLV), and the improved new RTase has higher template affinity and lower RNase H activity, thus enabling it to read through complex secondary structure templates and reverse transcript long fragment cDNA in the synthesis process of the first strand of cDNA. The 5×TIANScript II RTase Buffer provided in the product has been carefully optimized, which not only ensures the high-efficiency enzyme activity of the new RTase, but also widens the input range of RNA template amount, making the reverse transcribed cDNA have better quality for subsequent experimental analysis.
• Features■ High enzyme activity and efficiency: High reverse transcriptase activity and good compatibility in subsequent experiments.■ Wide substrate range: Suitable for all RNA, especially RNA templates with complex secondary structures.■ Long RT length: The synthesis of the cDNA first strand can reach 12 kb.■ Simple operation: Simply add the required components in one step without adding any reagent during the operation.
• Applications■ Synthesis of cDNA first strand.■ Construction of cDNA library.■ One-step RT-PCR.■ RACE analysis.

TIANScriptⅡ RT Kit

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