TIANSeq DNA Fragmentation Module-GNG305

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Efficient and rapid enzyme-based fragmentation of double-stranded DNA

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• StorageThis kit should be stored at-25~-15°C.
• DescriptionTIANSeq DNA Fragmentation Module is a kit designed for the construction of high-throughput sequencing platform library, which is used for fragmentation of large fragment DNA before library construction. The module adopts the principle of time-dependent enzymatic reaction to randomly cut double-stranded DNA into 200~500 bp fragments according to different reaction time. The obtained fragments are blunt-ended double-stranded DNA fragments, the 5′ end group contains 5′-P, and the 3′ end group contains 3′-OH, so that subsequent dA-tailing or adapter ligation can be directly performed. Random fragmentation of double-stranded DNA by TIANSeq DNA Fragmentation Module shows that the product has no base preference, and the sequencing coverage rate of different types of fragmented DNA is consistent with that of mechanical shearing.
• Required ReagentsTIANSeq Fast Ligation Module (Cat# 4992354/4992355)TIANSeq Size Selection DNA Beads (Cat# 4992358/4992359)
• Features■ No special instruments and equipment are needed. The double-stranded DNA can be easily and rapidly fragmented through enzymatic reaction.■ Flexible adjustment of DNA fragmentation length by simply adjusting fragmentation time.■ Efficient reaction: Fragmentation of 1 ng~1 μg DNA samples can be completed in a single reaction system.
• ApplicationsPrepare fragmented double-stranded DNA for NGS library construction

Publication

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• New genetic variations discovered in KRAS wild-type cetuximab resistant chinese colorectal cancer patients.Molecular carcinogenesis2020-07-07 Read ArticleAbstract: Clinical Cancer Research Center; Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University; Nanjing; China Department of Cell Biology; Nanjing Medical University; Nanjing; China Department of Chemotherapy; Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University; Nanjing; China. Molecular Carcinogenesis.. 2020;1??14. wileyonlinelibrary.com/journal/mc ?? 2020 Wiley Periodicals; Inc. | 1 Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University; Nanjing; China 2Department of Cell Biology; Nanjing Medical University; Nanjing; China 3Department of Chemotherapy; Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University; Nanjing; China Correspondence Jianzhong Wu; Clinical Cancer Research Center; Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University; Nanjing; Jiangsu Province 210009; China.
• The somatic mutation landscape of Chinese Colorectal CancerJournal of Cancer2020-01-01 Read Article.. sizes distributed between 200 and 300 bp using TIANSeq DNA Fragmentation Module Kit (Tiangen; China).

TIANSeq DNA Fragmentation Module

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