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Efficient preparation of RNA transcriptome sequencing library

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• Storage■ TIANSeq rRNA Depletion Kit (H / M / R) (4992363/4992364/4992391) should be stored at -20°C, avoid repeated freezing and thawing.■ TIANSeq Single-Index Adapter (Illumina) (4992641/4992642/4992378) should be stored at -20°C, avoid repeated freezing and thawing.■ TIANSeq Size Selection DNA Beads (4992358/4992359) should be stored at 2-8°C.
• DescriptionThe TIANSeq Stranded RNA-Seq Kit (Illumina) is a dedicated kit for the construction of strand-specific transcriptome libraries for the Illumina high-throughput sequencing platform. RNA library can be rapidly constructed through a fast one-tube operation process. After the synthesis of double-strand cDNA, the end repair and dA-tailing can be completed in one step, and the product can be directly used for adapter ligation without purification. In addition, this kit adopts specially designed high-fidelity and high-efficiency polymerase, and the enriched PCR products have high fidelity with no base bias.The input sample for this kit is rRNA-removed total RNAs (mRNA and other non-coding RNAs) or mRNA directly isolated from the total RNA. The initial template amount of total RNA samples is 10 ng-1 μg, and that of mRNA sample is as low as 1 ng. Application: It is suitable for the construction of RNA library for Illumina High-throughput Sequencing Platform.Sample input amount: 10 ng-1 μg total RNA. As low as 1 ng mRNA of animals, plants and fungus.
• Features■ Strand-specific library can be constructed for mRNA and non-coding RNAs (such as lncRNA) other than rRNA.■ It is easy to operate and can fast construct the RNA library.■ The high conversion rate of library is suitable for the efficient conversion of ultra-low initial sample input.■ The PCR enrichment process has high fidelity, with no base bias.
• ApplicationsApplicable for the targeted mRNA library and lncRNA library for Illumina sequencing platform.

TIANSeq Stranded RNA-Seq Kit (illumina)

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